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One of the most basic techniques of molecular biology to study protein function is molecular cloning. In this technique, DNA coding for a protein of interest is cloned using polymerase chain reaction (PCR), and/or restriction enzymes into a…

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One of the most important molecular biology methods is the polymerase chain reaction (PCR). It is used to exponentially amplify a specific segment of DNA for various downstream applications like DNA cloning (genetic engineering), gene…

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